8th Annual Bioprocessing Summit Europe

Speaker: Ivana Petrović Koshmak, Head of Upstream Process Development, Sartorius BIA Separations
Date and Time: March 20, 2025 (Day 2, Gene Manufacturing Track) | 12:10 PM
Title: Harnessing Chromatography and Ultracentrifugation Breakthroughs for AAV Capsid Ratio Assessment
Abstract:
Analytical tools for assessing empty and full AAV capsids often involve trade-offs among accuracy, precision, and resolution, balanced against time, skill level, and financial resources. Reproducibility challenges across different laboratories, frequently observed with sensitive equipment and varying protocols, further complicate this landscape. Consequently, chromatography is increasingly recommended for its robustness and ease of implementation from R&D to QC labs.

This talk explores the use of the PATfix AAV Switcher to optimize AAV production and enable in-process monitoring during production batches. We compare two bioreactor-scale production runs to assess upstream optimization’s effectiveness in reducing key impurities. Additionally, we discuss expanding the PATfix system with an ultracentrifugation (UC) module, offering a cost-effective and straightforward alternative to analytical ultracentrifugation for post-density gradient profiling of AAV materials.

About the event

Bioprocessing Summit Europe gathers over 750 industry and academic professionals to enhance the manufacture, quality, and control of next-generation biologics and genetic therapies. This 3-day, 14-track event is a key fixture in the European bioprocessing calendar, focusing on bioprocess R&D innovations and practical solutions for efficiency, speed, and cost. New for 2025, the summit includes a stream on process modeling and digitalization, with expanded content on ML/AI, advanced process control, and the latest in cell, gene, and RNA therapies.

Location: Barcelona, Spain + Virtual (CET)