BIA Separations

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monolith chromatography

A Guide to Monolithic Column Channel Sizes

A guide to choosing the right channel size of monoliths for biomolecules: pDNA, mRNA, AAV, LVV, bacteriophages, LNP, proteins, and more

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mrna

What’s Holding Back mRNA-LNP Therapeutics?

Solving challenges in the areas of purity, consistency, and monitoring technologies is crucial for the development of mRNA-LNP therapeutics.

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mrna

How Acidic Denaturation Facilitates dsRNA Removal

How to remove double stranded RNA for safer mRNA therapeutics, without special equipment and preserving the target molecule

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other

The Next Generation of Chromatography: Moving From Classic HPLC to Advanced Platforms

Advanced chromatography platforms enhance process development with integrated solutions beyond traditional HPLC systems.

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monolith chromatography

Transforming pDNA Purification: Insights from Christy Franco on VGXI's Monolith Technology Integration

VGXI's Christy Franco explains how monolith technology boosted pDNA purification yields from <10% to 70% and solved scale-up challenges

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aav

Optimizing AAV Upstream Process: High-Throughput Sample Preparation for Mass Photometer Analytics

Get fast and precise analysis of crude AAV harvest samples, using high-throughput sample preparation method for mass photometry.

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other

Key Differences Between CIM QA and CIM QA HR

Monoliths ensure that full capsids consistently elute at the same salt concentration across various scales, highlighting superior performance and reproducibility.

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aav

AAV Processing Made Simple: Your Guide to Empty and Full Separation Success

A simple guide to overcome AAV empty/full purification and analytics challenges while enhancing overall product quality.

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monolith chromatography

Using Multi-Well Plates When You Have Limited Samples

How to use just a part of the well-plate with a vacuum manifold, positive pressure manifold or centrifuge without wasting your sample

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aav

The Story of Citric Acid and Non-Reproducibility in Empty/Full AAV Separation: What Could Go Wrong?

Citric ions cause binding competition and magnesium chelation. Small adjustments in your DSP lead to better result.

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aav

Optimizing AAV Processes: Strategies for Effective Purification and Analysis

This blog post addresses the challenges in AAV purification and analytics, highlighting the scientific approaches to enhance product quality

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monolith chromatography

AAV8 Capture Purification: Comparing Ion-Exchange Monolith and Affinity Resin

Recent advancements in chromatography have significantly boosted AAV production yields. CIMmultus® SO3 monolithic column, a strong cation exchanger (CEX) for AAV capture purification, has shown a remarkable 30% increase in...

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