Achieving Scalable, High-Yield Plasmid DNA Purification Processes

Join this webinar to discover strategies for overcoming key bottlenecks in plasmid (p)DNA downstream processing and learn how to:

• Tackle scale-up challenges in pDNA production, including effective control over lysis and chromatographic separation at large scale.
• Maximize recovery and consistency at gram-scale while adhering to regulatory standards.
• Implement continuous plasmid purification to maintain pDNA quality.
• Utilize at-line analytics to monitor and optimize purification in real time.

Speaker:

Klemen Božič, Scientist, Process Development mRNA | pDNA at Sartorius BIA Separations

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Abstract:

Using real-world process data, the session will explore how to optimize each step—from lysis to sterile filtration—for maximum yield, purity, and regulatory compliance. The webinar will feature case studies demonstrating a fully integrated, end-to-end pDNA purification process achieving:

• >98% integrity and >64% process recovery of supercoiled pDNA
• >95% sc pDNA in lysate using automated in-line lysis
• >90% recovery rates for both AEX and HIC steps
• <4% loss in final UF/DF and sterile filtration

Plus, we demonstrate real-time process monitoring using at-line analytics to fine-tune performance and ensure quality.