Advancing EV Research: Analytical Chromatography Solutions for Efficient and Comprehensive Characterization of EV Preparations With Varying Complexity

Watch this webinar to learn about:

• Streamlining EV analysis by minimizing the use of multiple techniques.
• Efficiently obtaining particle size, protein content, and specific markers from a single chromatography experiment.
• Comparing Analytical Size Exclusion Chromatography (SEC) with traditional methods.
• Key considerations for establishing reproducible methods, including immunolabeling.
• Utilizing two-dimensional liquid chromatography for understanding nanoparticle heterogeneity.
• Tools and strategies for scientists and professionals to enhance EV research and applications.

Speaker:
Darja Božič, Project Manager, Sartorius BIA Separations

Abstract:

The field of extracellular vesicles (EV) has been increasingly empowered in recent decades, with significant advances in sophisticated analytical tools and the development of high-tech applications. However, the isolation and effective analysis of EV samples remain a substantial challenge, posing time and financial burdens that often limit the scope of studies. Comprehensive characterization of EV preparations using several complementary techniques often results in large sample consumption during downstream process development and control, reducing the amount of the final product available for higher-purpose assays.

Is it possible to streamline EV analysis by reducing reliance on multiple techniques and enhancing efficiency in downstream monitoring and quality control? Learn how we reduce the laborious multi-method evaluation of samples at early stages of process development through the efficient extraction of information on particle size, protein content, and specific markers provided by a single chromatography experiment using advanced detection methods such as light absorbance, fluorescence, and light scattering. The aim of the webinar is to highlight the strengths and limitations of Analytical Size Exclusion Chromatography (SEC) in comparison to the conventional orthogonal methods. Key factors to consider when setting up new methods, including ensuring reproducible immunolabeling, will be addressed. We will also introduce a two-dimensional liquid chromatography approach, providing deeper insight into the heterogeneity of biological nanoparticles of similar size.

This webinar is tailored for scientists and professionals eager to overcome the challenges posed by the diverse nature of EVs and the intricate biological matrices they inhabit. It is designed to support experts in setting up their own practical tools to advance EV research and therapeutic applications.