ANZ Biologics Festival
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Speaker: Blaž Bakalar, Business Development Manager
Time and Date: February 5 at 9:25 AM | Next Generation RNA Therapeutics Forum stream
Title: Nucleic acid purification strategies for capture and polish steps with a novel approach for dsRNA removal
Location: Melbourne, Australia
Abstract:
Nucleic acid-based therapeutics are advancing beyond COVID vaccine applications to encompass a broad spectrum of advanced treatments. This evolution involves varied nucleic acid formats like mRNA, self-amplifying RNA, and circular RNA, each posing unique purification challenges. A significant concern across these modalities is minimizing dsRNA impurities. We introduce strategies that enhance yields and purity across RNA types, focusing on innovative purification techniques for effective dsRNA removal.
Key Learnings:
- Importance of pDNA purity in dsRNA formation.
- Techniques for minimizing dsRNA during in vitro transcription.
- Overview of a novel purification method for dsRNA removal with examples across size-ranges.