ISEV 2025

We are excited to announce that we will be attending the ISEV 2025, where Darja will present a poster.

Poster Presenter: Darja Božič, Process Development Manager, Sartorius BIA Separations
Date and Time: 23 – 27 April, 2025
Poster  Title: Common Properties of Extracellular Nanoparticles from Various Sources: Utilizing Chromatography for a Comprehensive Characterization of Complex Samples

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Abstract:
Challenges in achieving high purity preparations and limitations in established analytics remain a rudimentary burden in studies of extracellular vesicles (EVs) and their biological roles. A good understanding of preparations’ composition is crucial for relating specific functionalities to different sample constituents. To enable a comprehensive insight into the composition of various EV preparations, a two-dimensional liquid chromatography (2D-LC) method was developed. The method provides a detailed sample profile, displaying sample components resolved by their size, and heterogeneity of extracellular nanoparticles (ENPs), resolved by their charge.
In the present study, samples of different origin (natural biological samples and cell cultures) and varying complexity (from clarified cell-conditioned media to various EV-enriched preparations) were analysed using this approach. CD9, CD63 and/or CD81 were targeted as one of the most common EV-markers.

About Conference: 

ISEV is the Largest Community of Extracellular Vesicle Researchers in the World. They are the leading professional society for researchers and scientists involved in the study of extracellularly secreted vesicles (EVs, including exosomes and other types of vesicles). ISEV ‘s nearly 2,000 members enjoy great benefits, including discounted publishing rates in Journal of Extracellular Vesicles and Journal of Extracellular Biology and reduced registration to the ISEV Annual Meeting.

Location: Vienna, Austria