Webinar with VGXI: Optimizing Plasmid DNA Purification
webinar2025
Join us for a webinar with VGXI to explore advanced strategies for optimizing plasmid DNA purification. Learn how to:
- Enhance robustness and improve contaminant profiles, including genomic DNA and isoform homogeneity, with in-line/continuous lysis
- Remove host cell RNA and proteins while concentrating multiple pDNA isoforms (OC, SC, multimers) in the eluate
- Implement selective hydrophobic interaction chromatography (HIC) for purity analysis to monitor pDNA quality
- Ensure compliance with regulatory standards on pDNA quality
Speakers:
Christy Franco
Director, Commercial Operations at VGXI
Jasmina Puc
Product Manager for Nucleic Acids at Sartorius BIA Separations
Abstract:
This webinar will delve into the specific technical challenges faced during pDNA production, to maximize the yield and purity of the final product while maintaining compliance with regulatory standards. Discover how tools such as Design of Experiments (DOE) and chromatographic analysis can streamline purification processes, enhance recovery rates, and ensure consistent production quality at gram scale. Our expert speakers will discuss the latest advances and trends in pDNA production, including scalable and robust process designs that meet the growing demand for high-quality pDNA in therapeutic applications. They will also draw on their extensive knowledge and practical experience to present case studies including real-life data on optimization of pDNA yield and quality.