World Vaccine Congress Europe
conferenceListen to our presentation
Speaker: Mojca Tajnik Sbaizero, Head of Product-Application Area, Process Development for Viral Vectors and Vaccines, Sartorius BIA Separations
Time and Date: Wednesday, October 30, 2024 | 3:30 PM
Title: Monoliths as an Effective Tool in Vaccine Production – Showcases of Fast And Efficient Virus Purification
Abstract:
Maintaining the final product’s purity and safety is a critical challenge in biomanufacturing, especially when maximizing production efficiency, such as vaccine production. Modernizing existing production processes is an open challenge, as higher productivity should not compromise product safety.
Recombinant vaccines are often purified by chromatography due to its scalability, purity and high recovery rates. It also enables process intensification by introducing fewer, more efficient purification steps and reducing costs. Convective Interaction Media (CIM®) monoliths are proven tools for the purification of large biomolecules, such as viruses, virus-like particles, and nucleic acids, commonly used in vaccines, cancer immunotherapy, or gene therapy.
In this talk, three different cases will be presented. For Influenza and Newcastle disease virus purification, we developed one-step purification from clarified harvest to formulated product using CIMmultus® SO3 column. This approach achieved extreme column capacities, fast flow rates and high overall recovery (>80%), leading to higher productivity compared to traditional methods. The presentation will also cover the DoE optimization of lentivirus purification using CIM® monolithic 96-well plates, which increased the infectious lentivirus process recovery to approximately 60% with the CIM® QA column.
In all cases, monolith chromatography significantly enhanced overall productivity and produced high-purity products.
About the event
Event will continue to cover vaccine progress in: Infectious Diseases, Cancer Immunotherapy, One Health and Veterinary Vaccines, Clinical Development, Manufacture, Safety, Supply, Access, Emerging Infectious
From basic research to commercial manufacture, this one meeting covers the whole vaccine value chain where science, government and manufacturers all come together to create ground- breaking progress. If you are involved in vaccines or new to it because of the pandemic, this is the most important meeting you will be part of in 2024.
Location: Barcelona, Spain