Seminars & Webinars

Resources

Optimizing AAV and LV Purification and Analytics: Accelerated High-Quality Processes using CIM® Monoliths and PATfix®

Explore Hana Jug's presentation from BPI East 2025 on optimizing AAV and LV purification using CIM® Monoliths and PATfix. Learn how these tools ensure high purity, effective impurity removal, and comprehensive sample analysis, enhancing efficiency and quality in biopharmaceutical production.

Orthogonal PAT Analytics – Essential to Enable Robust Production of Viral Vectors and LNPs

Explore the challenges and innovative solutions in lipid nanoparticle (LNP) drug delivery, focusing on targeted LNPs (tLNPs) and the use of PATfix LNP Switcher for robust analytics and process development.

Advancing Nucleic Acid Therapeutics: Scalable Strategies for pDNA, mRNA, and LNP Purification Supported by Rapid At-Line Analytics

Next-Gen LNP Pharmaceuticals Analytics: Intact Chromatography for Comprehensive Characterization

Discover innovative chromatography techniques for LNP pharmaceuticals, focusing on comprehensive characterization and process optimization.

A Novel Approach for dsRNA Removal Using Oligo dT Affinity Chromatography by Targeting Hydrogen Bond

Comprehensive Evaluation of RNA Integrity, Nanoparticle Properties, and Lipid Composition in RNA LNP

Achieving Scalable, High-Yield Plasmid DNA Purification Processes

Explore our recorded webinar on overcoming bottlenecks in plasmid (p)DNA downstream processing. Learn optimization strategies for lysis to sterile filtration, achieving high yield, purity, and compliance. Discover case studies showcasing integrated pDNA purification with real-time process monitoring and at-line analytics for enhanced performance and quality assurance.

Virtual Event: Addressing Challenges in pDNA, mRNA, and LNP Development, CMC, and Production

Access the recordings of our virtual event addressing challenges in pDNA, mRNA, and LNP development, CMC, and production. Gain insights from leading experts like Dan Peer and Jacek Lubelski on gene expression modulation, RNA delivery, and LNP manufacturing. Explore scalable purification workflows, innovative approaches to gene editing, and advanced analytical techniques for therapeutic applications. Enhance your understanding of nucleic acid delivery and LNP complexity with practical solutions for overcoming CMC hurdles.

Webinar with Refeyn: AAV6 Capsid Optimization with CIM SO3 Plates & Mass Photometry

Speakers: Hena Divanovic, Application Scientist at Refeyn Marko Narobe, Product Manager for High Throughput Solutions at Sartorius BIA SeparationsWatch the webinar to learn how to: The use of CIM SO3...

Method Optimization & High Throughput Purification for mRNA and rAAV

Speaker: Marko Narobe, Sartorius BIA SeparationsAbstract: The downstream processing of virus particles, vesicles, RNAs, plasmids and other forms of DNA, contains multiple interdependent steps, each requiring optimization for best results....

The Importance of Viral Clearance and Characterization in Gene Therapy Products

Speakers: Ya-Chen Chang, Regenxbio Akunna Iheanacho, Texcell Katelyn Pritchard, Texcell Maja Leskovec, Sartorius BIA Separations Maja Leskovec, Head of Process Development Viruses at Sartorius BIA Separations, presented "High Resolution Chromatography...

Advancing EV Research: Analytical Chromatography Solutions for Efficient and Comprehensive Characterization of EV Preparations With Varying Complexity

Speakers: Darja Božič, Project Manager, Sartorius BIA SeparationsAbstract: The field of extracellular vesicles (EV) has been increasingly empowered in recent decades, with significant advances in sophisticated analytical tools and the...

Optimizing Plasmid DNA Purification | Webinar with VGXI

Speakers: Christy Franco, Director, Commercial Operations at VGXI Jasmina Puc, Product Manager for Nucleic Acids at Sartorius BIA SeparationsWatch the webinar to learn how to: Enhance robustness and improve contaminant...

Enhancing Manufacturing Efficiency and Quality Through Optimized Lysis in Plasmid DNA Extraction

Speakers: Robin Pelizzari, Scientist pDNA small scale DSP Development, mRNA Center of Excellence at Sanofi Vaccine, France Matevž Korenč, Product Manager at Sartorius BIA Separations, SloveniaWatch the webinar to learn...

Accelerate Your AAV Process Development With a New Wave Analytical Tool

Speaker: Andreja Gramc Livk, Sartorius BIA SeparationsAbstract: Adeno-associated viruses (AAVs) are widely used vectors for gene therapies mainly due to their favorable safety profile and high transduction efficiency to various...

AAV Capsid Analysis and Quantification With Chromatography and Ultracentrifugation-Based Techniques

Speakers: Andreja Gramc Livk, Head of Process Analytics, Sartorius BIA Separations Sebastijan Peljhan, Head of Basic Research, Sartorius BIA SeparationsAbstract: This webinar will explore empty-to-full AAV capsid ratio determination using...

In-Line Lysis System: The Easiest Alternative to Batch Alkaline Lysis

Speaker: Matevz Korenc, Product Manager, Sartorius BIA SeparationsAbout the Speaker After receiving a PhD in Biochemistry, Matevz Korenc continued as a team leader in Knauf insulation, where he managed the...

Advancing LNP Characterization for Drug Delivery: NeoVac

Speakers: Blaž Bakalar, Product Manager, PATfix® Platform, Sartorius BIA Separations Dan Peer, Chairman, NeoVac Tristan Kovačič, Scientist, Process Analytics Development, Sartorius BIA Separations Abstract: Lipid nanoparticles (LNPs) are cell-specific delivery...

Optimizing LNP Formulations: Leveraging Monolithic Columns for Precise Insight and Improvement Strategies

Watch this webinar to learn about: How to capitalize on the advantages of scalable methods for separation of different biomacromolecules Understand the benefits of highly tunable chromatographic methods Discover how...

FastFacts: PATfix pDNA Platform for Monitoring pDNA Purification Process

Watch the video or read the poster to learn: The importance of pDNA as an enabling product in the production of mRNA, AAV, and other therapeutic vectors How the PATfix™...

Optimizing Lentiviral Purification: Enhancing Chromatography With Monoliths

Speaker: Luka Bevc, Manager Product|Application Area, Sartorius BIA SeparationsAbstract: Lentiviral vectors (LVVs) can effectively deliver up to 10 kb of target genes and can infect non-dividing cells, making them a...

Quality by Design in mRNA Manufacturing: At-Line Monitoring Coupled With DOE for Higher IVT Yield and Efficiency of Purification

Speaker: Rok Sekirnik, Head of Process Development for mRNA and pDNA, Sartorius BIA SeparationsAbstract: Due to the catalytic, multicomponent nature of the IVT reaction, optimization is a multi-factorial problem, ideally...

Enhancing mRNA Production with Advanced Analytics: WACKER Biotech

Speakers: Blaž Bakalar, Product Manager, PATfix® Platform, Sartorius BIA Separations Michael Daume,Group leader, Downstream Process Development for Nucleic Acids, WACKER BiotechAbstract: Production of mRNA involves complex IVT mixtures, including target...

Accelerating AAV Process Development: NCSU BTEC

Speakers: Blaž Bakalar, Product Manager, PATfix® Platform, Sartorius BIA Separations Shriarjun Shastry (Arjun), Scientist II, Biomanufacturing Training and Education Center (NCSU BTEC)Abstract: Adeno-associated viruses (AAVs) are widely used vectors for...

Implementation of an Improved Platform for Downstream Processing of Plasmid DNA

Speakers: Justina Martinkiene, Scientist, Purification Sciences, AstraZeneca Klemen Božič, Process Development Scientist, Sartorius BIA SeparationsAbstract: Plasmid DNA (pDNA) represents the backbone of most cell and gene therapy production workflows. As...

Comparability of Five Alternative AAV9 Downstream Processes from Ultracentrifugation to Chromatography

Speakers: Aleš Štrancar, Executive Managing Director, Sartorius BIA Separations Joe Balleydier, Senior Vice President of Research and Development, Advanced Medicine Partners Mike Hatfield, Senior Director of Research and Development, Advanced...

Improving the Safety of Your LNP-mRNA Therapeutics and Vaccines by Utilizing Monolithic Chromatography

Speakers: Andreja Gramc Livk, Head of Process Analytics Tristan Kovačič, ScientistLipid nanoparticle (LNP) based therapeutics and vaccines have been at the forefront of the non-viral delivery since SARS-CoV-2 vaccines rolled...

Process is the Product: A New Analytical Tool for AAV Production Optimization and Monitoring

Speakers: Blaž Bakalar, Product Manager PATfix® Platform, Sartorius BIA Separations Ivana Petrović Koshmak, Head of Upstream Process Development, Sartorius BIA SeparationsAbstract: Chromatographic analysis of complex AAV presents challenges due to...

Mastering Monolith Chromatography with DOE Strategies for Lentiviral Vector and Exosome Purification

Speakers: Maja Leskovec, Head of Process Development Viruses Luka Bevc, Scientist Andrej Raspor, ScientistAbstract: Join us for an insightful webinar on the latest advancements in monolith chromatography for lentiviral vector...

Fast & Efficient rAAV Downstream Process Optimisation With CIM Monolithic 96-Well Plates

Speaker: Mojca Tajnik Sbaizero, Project Manager, Sartorius BIA SeparationsAbstract: rAAV downstream process using monoliths contains a series of steps that are mutually dependent. Therefore each step should be optimized for...

Fast In-Process Analytics to Enable Robust mRNA Manufacturing

Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Typical mRNA production process involves four key steps: 1) plasmid DNA (pDNA) expression in E.coli, linearization and pDNA purification, 2) in-vitro transcription...

From Process Development to Manufacturing: How to Successfully Optimize and Scale up the AAV Enrichment Step on Novel CIMmultus QA HR Line

Watch the presentation to learn about: Chromatographic purification of AAV Development of the enrichment step Improving resolution and reproducibility with the CIMmultus HR line Successful scale up Speaker: Rok...

Enabling Tools for Purification and Understanding Complex Biomolecules Structure

Speaker: Aleš Štrancar, Managing Director, Sartorius BIA Separations

Exosomes: New Tools for Industrial Purification and Process Monitoring

Speaker: Pete Gagnon, Sartorius BIA Separations

Cornerstone Technology for Purification of Clinical-Quality AAV

Cornerstone Technology for Purification of Clinical-Quality AAV.mpeg 39,75 MB

Analytical Methods: The Path to More Efficient Processes & Safer Gene Therapy Products

Speakers: Aleš Štrancar, Managing Director, Sartorius BIA Separations

A Cornerstone Platform for Industrial Purification of Exosomes

Speakers: Pete Gagnon, Sartorius BIA Separations

CIMmultus Monoliths – Tailored to Accelerate Process Development and Address Large Scale Manufacturing of ATMPs and Vaccines

Speakers: Ingo Nagler

CIMmultus Monoliths – Tailored to Accelerate Process Development and Address Large Scale Manufacturing of ATMPs like AAV, pDNA, mRNA or Exosomes

Speaker: Ingo Nagler

Purification of Single-Stranded mRNA: The Toolbox for the Next Generation

Abstract: Messenger RNA is poised to become a major contributor in the fields of gene therapy and vaccines. Making this a practical reality requires purification technology that accommodates its unique...

Chromatin (DNA) Removal From Harvest Before an AAV Capture Step Greatly Improves Robustness, Purity, and Yield of the Overall Downstream Process

Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Adeno associated virus (AAV) is the leading vector in the field of gene therapy because of its low toxicity, good overall safety...

Platform Purification of Clinical Quality Bacteriophage

Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Many of the most compelling applications of bacteriophages involve human therapy, some pertinent to gene therapy, others to antibiotic replacement. These applications...

High Yield mRNA Production Process from E.Coli to Highly Pure mRNA

Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Established processes for pDNA manufacturing include purification steps designed to enrich the supercoiled (sc) isoform. Homogeneous supercoiled isoform improves cell transfection efficiency...

Ways to Improve Empty, Full and Damaged Capsids Separation

This webinar features: Buffer optimisation and proper column ligand selection (CIM QA, CIM PrimaS or new CIM EF) allows for >90% full capsid production Introduction of Specimen allows for production...

Chromatographic Tools for High-Yielding mRNA Production Process & Integrated Production Process of mRNA From E. Coli to Highly Purified mRNA

Our new Head of Process Development for mRNA and pDNA purification, Rok Sekirnik, presented our Chromatographic tools for high-yielding mRNA production process as workshop. Our CEO, Ales Štrancar presented our...

Efficient, Robust and Scalable Purification of Covid Vaccines Based on pDNA, mRNA, Adeno and VLP Technologies

In-Process Analytics of AAV Vector Capsid Production

Speaker: Ivana Petrović Koshmak, Head of Upstream Process Development, Sartorius BIA SeparationsAbstract: In-process analytics of vector capsid production is a critical optimization target in development of AAV-based gene therapy products....

Unlock the Potential of the mRNA Vaccine Platform with Integrated Production Processes from E. coli to Highly Purified mRNA

Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: A new purification approach starting from E.Coli all through to mRNA production is presented here. This new approach integrates a pDNA linearisation...

From E. coli to mRNA Drug Substance – Development of Analytical and Purification tools for a scalable mRNA production process

Speaker: Rok Sekirnik, Sartorius BIA SeparationsAbstract: This talk presents case studies of leveraging the power of at-line HPLC monitoring for process development to increase purity and yield of mRNA production....