Comparison of Cation-Exchange Monoliths: CIM SO3 and Multimodal CIM SO4 Monoliths Using a Standard Protein Mixture

This application note compares CIM SO3 and CIM SO4 cation-exchange monoliths. Both are strong cation exchangers, but SO4 ligand provides additional hydrophobic and hydrogen-bonding interactions. Under identical chromatographic conditions, SO4 showed increased retention of cytochrome c and lysozyme, consistent with multimodal behavior.

While with CIM SO3 higher lysozyme capacity was achieved at low salt, CIM SO4 maintained higher lysozyme capacity at > 200 mM NaCl concentration, allowing efficient operation under elevated salt conditions. Due to different ligands characteristics, it is recommended to refine CIM SO3 methods for CIM SO4 to exploit multimodal interactions and achieve optimal chromatographic performance.

*The CIMmultus SO4 discussed in this study is not yet available for commercial sale.  If you are interested in beta-testing the SO4 column, please contact azbe.znidarsic@biaseparations.com

Comparison of Cation-Exchange Monoliths: CIM® SO3 and Multimodal CIM® SO4 Monoliths using a Standard Protein Mixture.pdf 109,59 KB