Integrating Upstream and Downstream Processes for Enhanced Purity and Yield of Adenovirus 5

The document describes a streamlined adenovirus (AdV) production process that integrates optimized upstream methods with a scalable downstream purification strategy.

Using suspension HEK cells and a single chromatographic step on a CIMmultus QA column, the process achieved high yields and ~5-log impurity reduction while maintaining robustness and reproducibility. Advanced analytical tools like PATfix enabled real-time monitoring, making the approach suitable for gene therapy and vaccine applications