Purification of rAAV With CIM SO3 Monolithic 96-Well Plate

CIM® Monolithic Well Plates offer robust and reliable screening of multiple chromatographic conditions or high-throughput purification of large biomolecules like viruses and nucleic acids. Presented method describes quick and simple high-throughput purification of crude rAAV lysates using CIM® SO3 0.2 mL Monolithic 96-well Plate (2 μm channels). Its main advantage is purification of low volume rAAV upstream samples (such as 1 mL of lysate) that can be then used for various analytics that require certain purity and concentration: mass photometry for capsid species, PATfix E/F or SEC-MALS or other. It is of particular use when many crude lysates should be purified simultaneously, such as rAAV capsid optimizations, library screening, transfection optimization or simply when small volume of purified lysate is needed for downstream analytics.