Method Guides
Resources
Quick Guide for Purification of mRNA With CIM® Octa Oligo dT Columns
This method guide describes the use of CIM® Octa Oligo dT columns for the purification of mRNA. The document is designed to assist in use of the columns for automated...
Full AAV Enrichment with CIMmultus PrimaS® HR
CIMmultus PrimaS® HR for AAV enrichment, combining hydrogen bonding with weak anion exchange, ensuring high reproducibility, advanced impurity removal, and high recovery rates for all AAV serotypes.
Purification of mRNA With CIM® Oligo dT Monolithic 96-well Plate
Optimize your mRNA purification with our comprehensive guide on CIM® Oligo dT Monolithic Well Plates. Designed to assist in screening multiple mRNA constructs, this document provides detailed instructions for experiment setup, sample preparation, and buffer usage. Explore chromatographic conditions for seamless transfer to preparative CIMmultus® Oligo dT columns, with insights on plate size variants for flexible and adaptable purification processes.
Chromatographic Purification of LNP-based Formulations with CIMmultus® OH
Discover the chromatography-based purification of lipid nanoparticles (LNPs) using CIMmultus® OH monolithic columns. This method offers fast flow rates, minimal shear stress, and combines buffer exchange, ethanol removal, and LNP concentration in one run. Achieve higher recoveries, enhanced stability, and uniform size distribution compared to traditional techniques like TFF and dialysis, while reducing costs through column reuse.
CIM® Swiper for Comprehensive RNA Purification
CIMmultus Swiper is a chromatography column designed for efficient nucleic acid purification in a near-neutral pH range. Follow this method guide to purify mRNA
AAV Capture Step with CIMmultus SO3
CIMmultus SO3 is a monolithic chromatographic column with SO3 (Sulfonate), a strong cation-exchanger that binds positively charged molecules and repels negatively charged ones across all pH levels. It effectively binds...
Purification of mRNA With CIMmultus Oligo dT
The ligand in CIM Oligo dT18 is an 18-mer deoxythymidine chain covalently immobilized on the monolith with a carbon linker. RNA with a poly(A) tail binds on the ligand by...
Purification of mRNA With CIMmultus C4 HLD
The ligand in CIM C4 HLD monoliths is a hydrophobic butyl group. RNA binds on the column under hydrophobic conditions. The majority of DNA and short transcripts elute earlier than...
Purification of mRNA With CIMmultus SDVB
CIM SDVB is a polystyrene divinylbenzene monolith used for reversed phase (RP) purification of large biologics. It is used in purification of nucleic acids for size fractionation. In addition to...
Basic Principles for the Best Results With pFix5 Standard
This procedure describes the experimental materials and methods to perform analysis for standard curve with the pFix5 standard.
Basic Principles for the Best Results With pFix15 Standard
This procedure describes the experimental materials and methods to perform analysis for standard curve with the pFix15 standard.
Basic Principles for the Best Results With mFix4 mRNA Standard
mRNA standard can be used for three complementary analytics: Oligo dT (affinity chromatography with CIMac Oligo dT column), PrimaS (bimodal anion exchange and hydrogen bonding with CIMac PrimaS column) and...
AAV Full Capsids Enrichment With CIMmultus QA HR
CIMmultus QA HR column is anion exchange chromatography monolithic column. One of its main applications is enrichment of AAV full particles. Empty and full AAV capsids have small difference in...
Specimen QA HR Method Guide
Specimen unit is small volume device shipped together with CIMmultus QA HR chromatography column. Specimen use is not a prerequisite for running the CIMmultus QA HR column, but it can...
Enrichment of Full AAV With CIMmultus PrimaT
CIMmultus® PrimaT is a monolithic chromatography multimodal column, mainly used to enrich full AAV enrichment. Separation is based on weak anion exchange, H-bond and metal affinity coordination interactions with the...
Purification of rAAV With CIM SO3 Monolithic 96-Well Plate
CIM® Monolithic Well Plates offer robust and reliable screening of multiple chromatographic conditions or high-throughput purification of large biomolecules like viruses and nucleic acids. Presented method describes quick and simple...
Purification of mRNA With CIMmultus PrimaS
The ligand in CIM PrimaS contains a series of hydrogen donors and hydrogen acceptors, combined with a weak anion exchange character. Its hydrogen bonding ability makes its selectivity entirely distinct...
Immobilization Procedures for Aldehyde Monolithic Columns
This procedure applies to CIMac Analytical Columns (0.1 mL), CIMmic, and CIMmultus Columns (1 mL and larger) of aldehyde (ALD) activation chemistry.
Immobilization Procedures for CDI Monolithic Columns
This procedure applies to CIMac Analytical Columns (0.1 mL), CIMmic, and CIMmultus Columns (1 mL and larger) of carboxy imidazole (CDI) activation chemistry.
Immobilization Procedures for Epoxy Monolithic Columns
This procedure applies to CIMmultus Columns (1 mL and larger) of Epoxy (XY) activation chemistry.
Immobilization Procedures for HDZ Monolithic Columns
This procedure applies to CIMac Analytical Columns (0.1 mL), CIMmic, and CIMmultus Columns (1 mL and larger) of hydrazide (HDZ) activation chemistry.