Posters

Resources

Optimization of Preparative-Scale mRNA Capture Using Small-Scale Oligo dT Affinity Monoliths

Optimize mRNA dynamic binding capacity using CIMmic Oligo dT discs, which couple oligo-deoxythymidilic acid probes to monolith surfaces for selective mRNA binding. This study highlights the reduction in sample consumption and increased DBC from 1.9 mg/mL to 6.8 mg/mL through flow rate and buffer composition optimization, making CIMmic discs invaluable in early development.

Modulating and Understanding Retention of Proteins on Chromatographic Support by Changing Cation-Exchanging Ligand

This study compares protein separation using CIM SO3 and CIM SO4 monolith cation exchangers, highlighting differences in elution under an ascending salt gradient. Through thermodynamic analysis, it explores how sulfonate and multimodal sulfate ligands affect retention and adsorption of lysozyme and cytochrome c, demonstrating CIM SO4's enhanced selectivity and potential as a tool for challenging protein separations.

Accelerating AAV Processing: Scalable, Serotype-Agnostic Process Eliminating 2-steps (DNase and TFF) and Optimized for Physiological-Like Conditions

This study proposes a streamlined alternative to traditional AAV downstream processing, focusing on reducing the number of steps while simultaneously improving both recovery and process efficiency. We demonstrate a novel approach comprising just five integrated steps shown to work on various serotypes (AAV2, AAV8, AAV9).

Efficient Removal of Activated Coagulation Factor XI (FXIa) from Cohn Fraction II Paste Using Mixed Mode Ligand

Factor XI (FXI) is the zymogen of a serine protease, Factor XIa (FXIa). It is a 160 kDa protein, with an average plasma concentration of 3 to 7 µg/ml. Its...

Monolith Based Chromatography Is a Suitable Tool to Prepare and Analyze E. coli – Outer Membrane Vesicles

Explore the collaborative study with NecstGen on developing a scalable, GMP-ready DSP platform for lentiviral vector (LV) gene therapies. Utilizing Sartorius BIA Separations' CIM QA and DEAE Monolithic 96-well plates and CIMmultus QA columns, the study achieved significant improvements in DSP yields, reducing manufacturing costs. Discover how Design of Experiments (DOE) methodologies enhanced process understanding and LV recovery, optimizing purification efficiency and product quality for cost-effective gene therapy solutions.

Collaborative Development with NecstGen: Scalable GMP-Ready LV DSP Platform

PATfix® pDNA Platform and PCR Methods as Orthogonal Analytical Tools for Quantification of pDNA

Poster presents a comparison of three orthogonal methods—PATfix® pDNA Platform, qPCR, and dPCR—for the quantification and characterization of plasmid DNA (pDNA) in gene therapy workflow. While PCR-based approaches provide total...

CIMmultus Oligo dT Affinity Column Based Extraction of mRNA From IVT Mixtures

The increasing demand for messenger RNA (mRNA) as a therapeutic product requires larger production scales and more efficient extraction techniques. In this poster, fast and efficient way to purify poly-adenylated...

Monitoring Orf Virus Production with PATfix® Analytical Method

Orf virus (ORFV) is a promising vaccine vector candidate, capable of inducing a strong, long-lasting immune response without integrating into the host genome. Its broad host range and ability to...

Optimizing AAV Purification: Comparative Evaluation of Three CIMmultus® Polishing Columns for Enhanced Vector Enrichment and Final Product Purity

The poster presents a comparative evaluation of three CIMmultus® polishing columns—QA HR, PrimaT, and PrimaS—in optimizing the purification of AAV vectors. Highlighting their unique strengths, the study reveals QA HR's...

High Throughput Screening (HTS) or Single-Column Approach for Improvement of Empty/Full Separation of AAV

Accelerate AAV development with a fast, reproducible polishing workflow that separates empty and full capsids across three scalable CIM® monolith formats - from 96 well plates to CIM® Octa and...

Enhanced Monitoring of AAV Downstream Processes Using Liquid Chromatography Approaches

In-process control of AAV downstream processes (DSPs) ensures process efficiency, product quality, safety and consistency . Using liquid chromatography (LC)-based methods such as size exclusion chromatography (SEC), cation exchange (CEX),...

Advancements in Lentiviral Purification: From High Throughput Screening to Scalable Solutions

Lentiviral vectors are being increasingly used as tools for stable integration of large gene inserts into the genomes of both dividing and non-dividing cells. With 3rd and 4th generation of...

Integrating Upstream and Downstream Processes for Enhanced Purity and Yield of Adenovirus 5

The document describes a streamlined adenovirus (AdV) production process that integrates optimized upstream methods with a scalable downstream purification strategy. Using suspension HEK cells and a single chromatographic step on...

End-To-End Process for Improved Purity, Combined With Innovative Analytics of LNP-Based Therapeutics

This poster presents a comprehensive approach to optimizing the mRNA-LNP production process, focusing on key steps from plasmid DNA expression in E. coli to encapsulating nucleic acids into lipid nanoparticles...

Monoliths for a Novel, Selective and High-Recovery Purification Process of LNP-Based Biopharmaceuticals

CIMmultus OH monolithic columns can be utilized as a chromatographic solution to purify LNPs. This process is used as a direct alternative to existing processes like TFF or dialysis, but...

Efficient rAAV8 Capsid Quantification in Upstream Process Development With Two-Dimensional Liquid Chromatography

Obtaining a high concentration and a significant proportion of full recombinant adeno-associated viruses (rAAV) capsids during production is important for the success of the entire AAV bioprocess. Analyzing complex upstream...

Immobilized Enzyme Reactors on Monoliths: A Platform For Efficient pDNA Linearization

Immobilized enzyme reactors (IMERs) on monolithic supports were developed for efficient plasmid DNA linearization, a critical step in mRNA production. EcoRI, NdeI, and HindIII were successfully immobilized, achieving comparable linearization...

Poster by Genethon | Full AAV8 Capsid Enrichment Step with the CIMmultus® PrimaT

Development of a Full Capsid Enrichment Step in the Purification Process of an AAV8 Product by the CIMmultus® PrimaT Monolith With CIMmultus PrimaT extremely consistent full AAV enrichment &...

Poster by Genethon | Full AAV9 Capsid Enrichment Step with the CIMmultus® PrimaT

Development of Full Capsid Enrichment Step in AAV Purification Process with CIMmultus® PrimaT Monolith CIMmultus PrimaT demonstrates efficient separation of empty and full AAV capsids even from AAV9 serotype....

Lentiviral Downstream Process Optimisation and Design of Experiments on CIM QA 0.05 mL Monolithic 96-Well Plates

Lentiviral vectors are being increasingly used as tools for stable integration of large gene inserts into the genomes of both dividing and non-dividing cells. With 3rd and 4th generation of...

Comparing Cation-Exchange Monolith and Affinity Resin for Chromatographic Capture and Purification of rAAV

This study evaluated the purification efficiency of the CIMmultus SO3 column compared to two commercially available affinity resins for Adeno-Associated Virus (AAV) purification. AAV material was prepared using two distinct...

Manufacturing Platform – From pDNA and mRNA to LNP With Multiple Nucleic Acids Loads

The typical mRNA production process is a complex sequence of four key steps, each crucial for ensuring the quality and efficacy of the final product. The process begins with the...

Comparability Study Between Ion-Exchange Monolith and Affinity Resin For Purification of AAV8

The main objective of every downstream process (DSP) for AAV is to achieve high recovery while delivering the purest, most potent product possible. The capture step in AAV gene therapy...

A Chromatographic Tool to Comprehensively Characterize Complex Novel RNA-LNP Drug Products

To address challenges in LNPs characterization, we have developed a method capable of simultaneously evaluating key parameters of the LNP formulations.

Common Properties of Extracellular Nanoparticles From Various Sources: Utilizing Chromatography for a Comprehensive Characterization of Complex Samples

Challenges in achieving high purity preparations and limitations in established analytics remain a rudimentary burden in studies of extracellular vesicles (EVs) and their biological roles. A good understanding of preparations’...

mRNA | saRNA Process Development With PATfix Analytical Platform

Recent advancements in mRNA-based vaccines and therapeutics have simplified production compared to traditional vaccines, enabling rapid large-scale output. The process involves in vitro transcription (IVT) and chromatographic purification, requiring effective...

Monolithic Columns for a High Recovery Purification Process of RNA-LNP Vaccines and Therapeutics

To address challenges in LNPs characterization, we have developed a method capable of simultaneously evaluating key parameters of the LNP formulations.

Advancing AAV Analytics: Post-DGUS Profiling as a Robust Tool for AAV Capsid Characterization

Density gradient ultracentrifugation (DGUC) is a well-established method for separating empty (E) and full (F) AAV capsids based on density differences. However, traditional DGUC is labour-intensive and lacks direct detection...

Monolithic Columns Enable Streamlined Downstream Processing and Analytics of Lipid Nanoparticles

Lipid nanoparticles are leading non viral carriers for therapeutics, offering versatility in encapsulating diverse payloads. Their manufacturing superiority over viral systems allows for modularity, speed, and scalability. However, this modularity...

2D-LC Evaluation of Complex Samples Containing Extracellular Vesicles

The aim of the study was the development of a robust and high-throughput method that would enable a comprehensive evaluation of extracellular vesicles (EVs) in complex samples. Our goal was...

Targeted Optimization of AAV8 Upstream Process for Enhanced Downstream Performance

Several recombinant AAV (rAAV)-mediated therapies have been approved so far, and many more are in clinical trials, yet developing an efficient rAAV process platform remains a challenge. First obstacle arises...

Isolation of RAE-1 From Murine Cells Based on Immunoaffinity Monolithic Chromatography

Immunoaffinity columns using antibodies as ligands against mammalian proteins could be used for different applications in protein expression control and, if a standard available, for direct protein quantification in complex...

Effective Endotoxin Reduction in Bacteriophage Samples Using CIMmultus Monolithic Technology

Endotoxins are robust and persistent impurity, which are native to majority of phage substrates. Two anion exchangers, CIMmultus PrimaS and H-Bond, were tested for their capacity for endotoxin removal in...

Grafting Chromatographic Monoliths With Charged Linear Polymers for Highly Productive and Selective Plasmid DNA Purification

Increased requirements for plasmid DNA (pDNA) in gene therapy and vaccination efforts brings the need for efficient large-scale production processes with high purity and productivity. Chromatographic purification of pDNA is...

High Reproducibility, Homogeneity, and Scalability of CIM® QA HR Chromatographic Monoliths Demonstrated by Separation of Empty and Full AAV Capsids

One of the key challenges in adeno-associated virus (AAV) viral vector manufacturing is effective and consistent separation of full AAV particles (containing the full recombinant DNA of the gene of...

Rapid Desalting of E. Coli Cell Lysate for CGE Analysis Using Monolithic Spin Columns

Neutralized E. coli cell lysate is a complex intermediate sample in the production process of plasmid DNA (pDNA) for use in vaccines and gene therapy. It contains various amounts of...

Chromatographic Separation of Full and Empty AAV8 Capsids

Adeno-associated virus (AAV) vectors of various serotypes are considered to have high potential for gene therapy applications. Currently, manufacturing of AAV vectors faces the challenge of co-production of incompletely formed...

Chromatographic Performance of Novel Polymetacrylate-Based Ion Exchanging Monoliths

Productivity of the downstream bioprocessing depends among others on the efficiency of chromatographic step. One of the crucial chromatographic parameters is dynamic binding capacity (DBC) for certain biomolecule. DBC could...

Optimizing a Separation of IgG Charge Variants Using Weak Cation-Exchanging Analytical Monolithic Column

The upstream and downstream monoclonal antibody (mAb) bioprocessing makes them susceptible to physical and chemical modifications. In the biotechnological production process of mAbs, structural variations may arise due to some...

Optimization of pDNA Downstream Bioprocessing Using Hydrophobic Monoliths in Sample Displacement Mode

Since plasmid DNA (pDNA) as a pharmaceutical product has stringent requirements of purity and efficacy, one or more chromatographic steps are often used in the downstream processing train. High ligand...

Large Volume CIM Chromatographic Monoliths – Scalability and Uniformity

To ensure the desired chromatographic characteristics of the CIM® monolithic column at large scales, monolith microstructure morphology, pore size distribution, porosity and surface ligand density should be uniform. To demonstrate...

Immunoaffinity Columns | Reproducible and Reliable Tool for Analytics and Purification

There are many cases, where a single protein needs to be purified from a complex sample. Such proteins manifest themselves as impurities, which can affect further analysis, either by causing...

Overcoming the Limitations of Large Scale Polymerization

Methacrylate monoliths (CIM monolithic columns) allow for very fast and efficient separations and exhibit very high binding capacities for extremely large bio-particles due to their large inner channel diameters and...

Preparation of Immunoaffinity Columns for Purification and Analytics of MULT-1 and RAE-1

Immunoaffinity columns using antibodies as ligands against mammalian membrane proteins could be used for different applications in protein expression control and, if a standard available, for concentration determination. Additionally these...

High-Speed Quantification of Immunoglobulin G – CIMac r-Protein A Analytical Column

CIMac™ r-Protein A Analytical Column is short bed, high performance monolithic column . Primarily is intended for fast, efficient, and reproducible qualitative and quantitative analyses of Immunoglobulin G (IgG). It...

Testing Different Activation Chemistries for IgG Immobilization Onto a Monolithic Support

Interactions between antibodies and their antigens are highly selective and therefore immensely popular for affinity chromatography. Consequently, numerous antibody immobilizations were performed on monolithic supports via different activation chemistries in...